The psychoactive substances are defined as substances that when they are consumed generate a set of conditions in mental processes, both cognitive as emotional (OIT, 1996). The consumption of them can reach in the people’s quality of life, normally in a negative way. One of the spaces in which the preceding can be seen explicitly is in the workplace, in which people, in addition to developing in a personal way, also affect the overall objectives of an organization (productivity, efficiency, quality, among others). The objective of the present research is to characterize the psychoactive substances consumption in manufacturing workers of the ZMG, doing an analysis by gender, in addition to a relation of that consume with their quality of work-life. The sample was conformed by 1264 workers, of which 35% (442) are women, and 64.8% (819) are men, plus three unidentified workers; about their ages, these goes from 15 years until the 73 years, with an average of 34 years (SD=11.6). About the main results, we found that the substances most consumed by women are the tobacco, the alcohol, the tranquilizers, and the marijuana, while men consume the same and in addition the cocaine