Meditation is one of the most used mind-body therapies in the world. Meditation also affects changes in the autonomic nervous system. In general, meditation has been considered to have a relaxation response, but recent studies have shown that the psychophysical effects of meditation vary with the type of meditation and the level of meditation practice. The purpose of this study was to investigate the effect of meditation experience on heart rate variability, which is the most representative physiological index of stress and autonomic nervous system change. A total of 39 healthy college students (15 males and 24 females) were enrolled in the study. experiment group(19) was experienced meditation from lectures and practices in University. and control group(20) was not experienced meditation anything. and all participants measured their HRV by recording inducing during meditating. Data are analyzed with multi-level Linear model. Results showed that SDNN was significantly increased in meditation in both groups and that the group experiencing meditation had a significantly higher SDNN increase than the group without meditation. This study is significant as a basic study on the effect of heart rate variability on meditation experience.